CRISPR / Cas9 method: The cutting edge technique
This simple and fast method of changing genes has revolutionized the scientific world. The CRISPR / Cas9 method allows very fast and specific genome editing.
This technique has already been used successfully in many model organisms (yeast, plant, zebrafish, Drosophila, nematode and mouse) and also works very well in human cell lines. As a result, it has great potential to be used as a therapeutic agent in the near future. You can find more information about this in our information center.
Do you want to try this method too? Find out from our partner abm Crispr / Cas9 Guide using the short and clear manual or, in a somewhat more detailed form, from our partner Genscript CRISPR Handbook . For even more information, there is also a video that explains the CRISPR / Cas9 method in detail.
Product overview from our partner abm (Applied Biological Materials Inc.)
Genome editing with the help of CRIPR / Cas9 requires two components in particular: an endonuclease and a guide RNA (gRNA).
abm offers a wide range of Cas9 stably expressing cell lines . These are available for all popular research models. Cell lines with a cumate-inducible system can also control expression and control it over time. All cell lines are pre-validated by qPCR and / or by Western blot . For successful genome editing, the gRNA must be introduced into the cell lines using viral transduction or transfection. They can then be checked for a gene knockout.
There is also the possibility of transfecting Cas9 proteins together with the gRNA directly into existing cells in order to edit the genome (available on request for AAV, lentivector, adenovirus and non-viral vector expression systems). This is a very quick method because there is no transcription or translation. The Cas9 proteins offered by abm can be used on many organisms without having to consider codon optimization or promoter compatibility. In addition, this type of CRIPR / Cas9 method does not require plasmids or viruses, and therefore excludes integration into the genome.
The negative controls are particularly important for every experiment . Abm offers vectors and viruses that are suitable for use in all expression systems. Here you will find an overview of all negative controls . Choose a suitable control for transduction with non-viral vectors or with lentiviruses, AAV and adenoviruses.
Product overview from our partner GenScript
GenScript also offers a comprehensive portfolio of products for the CRIPR / Cas9 method. Here you will find the detailed GenScript product overview .
GenScript’s Cas9 proteins can be used for a wide range of applications, such as in vitro gRNA cleavage, in vivo genome editing, reduction of off-target effects and for sorting and enrichment with FACs.
Would you like to make your gRNA yourself? GenScript offers you the right gRNA synthesis kit . Before the gRNA is introduced into the cells, it is possible to select the most efficient gRNA. GenScript offers you a GenCrispr gRNA screening kit that quickly and effectively enables you to identify the best target gRNA.
In addition to Cas9 proteins, GenScript also offers highly efficient Cas9 gRNA plasmid assembly kits. The selection ensures that almost 100% of the clones are positive. You can choose between linear and circular form. Although the linear form allows digestion and purification to be omitted, the circular form has the advantage that the vector can also be used for further experiments.
The traditional transfection methods are based on the introduction of DNA into cells. These methods are usually not optimized for protein or protein / RNA transfection. GenScript has developed efficient methods to successfully introduce the Cas9 protein and the gRNA into the cell. The GsLipo reagent-dependent transfection shows a very high efficiency and low toxicity.
Other interesting CRISPR / Cas9 products
Our partners Biotools and Novateinbio also offer interesting products for your CRISPR / Cas9 experiments. Biotools provides three different proteins for genome editing: wtCas9, eSpCas9 1.1 , AsCpf1 . These proteins show very high efficiency, specificity and no off-target effects. Novateinbio has a range of Cas9 proteins, antibodies and kits for use around the CRISPR / Cas9 method.