Category: Blog

Cusabio N-terminal 6xHis-Flag-tagged Recombinant

FLAG system The isolation and analysis of protein complexes remain a great technical challenge in functional proteomics research. Co-immunoprecipitation is the most widely used method for the isolation of protein complexes. The TAP (tandem affinity purification) methodology incorporates tandemly linked affinity tags onto a target protein to extract and isolate endogenous interacting proteins. Protein complexes…

By Bertha Cooper February 5, 2022 Off

Cusabio Tag-Free Recombinant

Abstract Affinity tags can interfere with various physicochemical properties and immunogenicity of recombinant proteins. In the present study, tag-free recombinant fusion protein encompassing the promiscuous T-cell epitope of tetanus toxoid [TT; amino acid (aa) residues 830-844] followed by linker dilysine and dog zona pellucida glycoprotein-3 (ZP3; aa residues 23-348) (TT-KK-ZP3) was expressed in Escherichia coli.…

By Bertha Cooper February 5, 2022 Off

Cusabio N-terminal 10xHis-SUMO-tagged Recombinant

Properties Quality level: 200 Recombinant: expressed in E. coli Rehearsal: ≥95% (SDS PAGE) Form: lyophilized powder Molecular weight: 27kDa Sent in: environment Storage temperature: −20°C Related Categories: Proteases General description SUMO proteases are a general class of enzymes that specifically remove the protein post-translational modification (PTM) known as a small ubiquitin-related modifier (SUMO), which belongs…

By Bertha Cooper February 5, 2022 Off