Laccase from the white rot fungus Coriolopsis polyzona was immobilized for the first time through the formation of cross-linked enzyme aggregates (CLEAs). Laccase CLEAs were produced by using 1000g of polyethylene glycol per liter of enzyme solution as precipitant and 200muM of glutaraldehyde as a cross-linking agent.
These CLEAs had a laccase activity of 148Ug(-1) and an activity recovery of 60.2% when using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as substrate. CLEAs formed by co-aggregation with bovine serum albumin (BSA) as a stabilizer showed lower laccase activity and affinity for ABTS than those without BSA. The CLEAs co-aggregated with BSA showed higher residual activity against a protease, NaN(3), EDTA, methanol and acetone.
The thermoresistance was higher for CLEAs than for free laccase and also higher for CLEAs co-aggregated with BSA than for simple CLEAs when tested at a pH of 3 and a temperature of 40 degrees C.
5-Iodotubercidin |
A3125-5 |
ApexBio |
5 mg |
EUR 168 |
Description: 5-Iodotubercidin (Itu) is a purine derivative and hence an inhibitor of adenosine kinase with an IC50 value of 26 nM [1]. Adenosine kinase is important in regulating the intracellular and extracellular concentrations of adenosine and hence diverse physiological actions of adenosine [2]. |
Thrombin Receptor Activator for Peptide 5 (TRAP-5) |
A1036-5 |
ApexBio |
5 mg |
EUR 102 |
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response. |
2',5'-Dideoxyadenosine |
2770-5 |
Biovision |
|
EUR 240 |
Teicoplanin A2-5 |
C4938-5 |
ApexBio |
5 mg |
EUR 1157 |
Description: Teicoplanin A2-5, derived from Actinoplanes teichomyceticus, is a glycopeptide antibiotic which produces a broad spectrum of antibiotic activity against gram-positive bacteria. |
5'-deoxy Thymidine |
C4692-5 |
ApexBio |
5 mg |
EUR 180 |
Description: 5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines. |
5-fluoro 203 |
C4114-5 |
ApexBio |
5 mg |
EUR 154 |
Description: 5-fluoro 203 (5F-203) is an antitumor agent and cytotoxic compound that acts as a potent AhR agonist [1][2][3]. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in regulating xenobiotic-metabolizing enzymes such as cytochrome P450. |
W-5 hydrochloride |
B6277-5 |
ApexBio |
5 mg |
EUR 116 |
5-Nonyloxytryptamine oxalate |
B6496-5 |
ApexBio |
5 mg |
EUR 154 |
5-Formylcytosine (5-fC) ELISA Kit |
MET-5102-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2283 |
5-Carboxylcytosine (5-caC) ELISA Kit |
MET-5103-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2283 |
AICA riboside, 5'-phosphate |
9416-5 |
Biovision |
|
EUR 153 |
Individual Reaction Mix 5 |
G065-5 |
ABM |
200 reactions |
EUR 167 |
Necrosis Inhibitor, Necrox-5 |
2229-5 |
Biovision |
|
EUR 615 |
5-Nitroso-8-quinolinol |
B2825-5 |
Biovision |
|
EUR 109 |
Secretin (5 - 27), porcine |
5-01917 |
CHI Scientific |
4 x 1mg |
Ask for price |
FAM azide, 5- isomer |
A8117-5 |
ApexBio |
5 mg |
EUR 166 |
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel. |
ROX azide, 5- isomer |
A8126-5 |
ApexBio |
5 mg |
EUR 583 |
Description: ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.Pure 5-isomer. |
TAMRA azide, 5- isomer |
A8129-5 |
ApexBio |
5 mg |
EUR 583 |
Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.TAMRA is often used as FRET acceptor for FAM fluorophore. |
5'-(N-Cyclopropyl)carboxamidoadenosine |
C4645-5 |
ApexBio |
5 mg |
EUR 128 |
5-trans U-46619 |
C4261-5 |
ApexBio |
5 mg |
EUR 538 |
Description: 5-trans U-46619 is a minor impurity (2-5%) which variably exists in most commercial preparations of U-46619 [1]. U-46619 is a selective thromboxane A2 agonist involved in inducing platelet aggregation and vascular smooth muscle contraction [2]. |
2',5'-dideoxy Adenosine |
C3993-5 |
ApexBio |
5 mg |
EUR 189 |
Description: 2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1]. Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells. |
2-Methyl-5-hydroxytryptamine hydrochloride |
2768-5 |
Biovision |
|
EUR 185 |
3,3',5'-Triiodo-L-thyronine |
B2017-5 |
Biovision |
|
EUR 229 |
[Pyr5]-Substance P (5-11) |
5-00288 |
CHI Scientific |
4 x 5mg |
Ask for price |
[Trp3, Arg5]-Ghrelin (1-5) |
5-00313 |
CHI Scientific |
4 x 5mg |
Ask for price |
beta-Casomorphin (1-5) (bovine) |
5-00475 |
CHI Scientific |
4 x 5mg |
Ask for price |
Cholecystokinin Octapeptide (1-5) (desulfated) |
5-00970 |
CHI Scientific |
4 x 5mg |
Ask for price |
HIV-gp41-Antigenic Peptide 5 |
5-01331 |
CHI Scientific |
4 x 1mg |
Ask for price |
Magnetic Beads (DNA) 5 mL |
P920-5 |
101Bio |
- |
Ask for price |
Angiotensin 1/2 (1-5) |
A1047-5 |
ApexBio |
5 mg |
EUR 119 |
Description: Angiotensin I/II (1-5) is a peptide (ASP-ARG-VAL-TYR-ILE) that contains the amino acids 1-5 and is converted from Angiotensin I/II.Angiotensin I is formed by the action ofreninonangiotensinogen, an ?-2-globulin with 12 amino acids. |
Angiotensin 1/2 (5-7) |
A1049-5 |
ApexBio |
5 mg |
EUR 119 |
Description: Angiotensin 1/2 (5-7), (C17H27N5O4), a peptide with the sequence H2N-Ile-His-Pro-OH, MW=365.43. Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure. |
3,3',5'-Triiodo-L-thyronine |
C5014-5 |
ApexBio |
5 mg |
EUR 134 |
Description: 3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TR? and TR? antagonist [1]. |
2'-O-Methylpseudouridine-5'-Triphosphate |
B7993-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 14023 |
2'-Fluoro-thymidine-5'-Triphosphate |
B8035-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 2059 |
Helioxanthin derivative 5-4-2 |
B4700-5 |
ApexBio |
5 mg |
EUR 683 |
Description: Helioxanthin derivative 5-4-2, an analogue of helioxanthin (ACH126447), is a small-molecule inhibitor of HBV, HSV-1and HSV-2 virus with EC50 values of 0.08 ?M, 0.29 ?M and 0.16 ?M, respectively [1]. |
5?-dihydro-11-keto Testosterone |
C4317-5 |
ApexBio |
5 mg |
EUR 586 |
Description: 5?-dihydro-11-keto Testosterone (11KDHT) is a full androgen receptor agonist comparable to dihydrotestosterone (DHT) [1][2][3]. |
Nickel NTA Magnetic Agarose Bead (5%) |
MagNTANi5-5 |
ABTBeads |
5ml suspension |
EUR 174 |
beta-Casomorphin (1-5) amide (bovine) |
5-00476 |
CHI Scientific |
4 x 5mg |
Ask for price |
cAMP Dependent PK Inhibitor (5-24) |
5-00922 |
CHI Scientific |
4 x 1mg |
Ask for price |
Kinase Domain of Insulin Receptor (5) |
5-01429 |
CHI Scientific |
4 x 1mg |
Ask for price |
Fmoc-2-amino-5-Methoxybenzoic acid |
5-04549 |
CHI Scientific |
1g |
Ask for price |
Fmoc-2-amino-5-Methoxybenzoic acid |
5-04550 |
CHI Scientific |
5g |
Ask for price |
7078D 5 PIPET 5ML STERILE STERILE |
7078D-5 |
CORNING |
25/pk |
EUR 217 |
Description: Disposable Pipets; Disposable Pipets, Serological, Misc |
Beta-Sheet Breaker Peptide iA?5 |
A1005-5 |
ApexBio |
5 mg |
EUR 102 |
Description: Beta-Sheet Breaker Peptide iA?5 (C33H43N5O8), with the sequence H-Leu-Pro-Phe-Phe-Asp-OH, has been shown to inhibit amyloidogenesis in rat brain models. |
2'-Fluoro-2'-deoxyadenosine-5'-Triphosphate |
B7959-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 2059 |
2'-Fluoro-2'-deoxycytidine-5'-Triphosphate |
B7961-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 2059 |
2'-Fluoro-2'-deoxyguanosine-5'-Triphosphate |
B7962-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 2059 |
2'-Fluoro-2'-deoxyuridine-5'-Triphosphate |
B7963-.5 |
ApexBio |
5x100 µl (100 mM) |
EUR 2059 |
3-bromo-5-phenyl Salicylic Acid |
C4516-5 |
ApexBio |
5 mg |
EUR 370 |
Description: Ki: 140 nM for AKR1C1; 1.97 ?M for AKR1C2; 21 ?M for AKR1C3 3-bromo-5-phenyl Salicylic acid is an AKR1C1 inhibitor. |
5-bromo-3-phenyl Salicylic Acid |
C4610-5 |
ApexBio |
5 mg |
EUR 319 |
5-(N,N-dimethyl)-Amiloride (hydrochloride) |
C3505-5 |
ApexBio |
5 mg |
EUR 180 |
Description: 5-(N, N-dimethyl)-Amiloride (hydrochloride), a derivative of amiloride, is an inhibitor of NHE1, NHE2, and NHE3. The Na+/H+ exchanger (NHE) is a protein that has been involved in intracellular pH homeostasis of many mammalian cell types. |
CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps) |
CBA-155-5 |
Cell Biolabs |
25 preps |
EUR 3245 |
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
5-Hydroxytryptamine (5-HT) ELISA Kit |
DLR-5-HT-Ge-48T |
DL Develop |
48T |
EUR 469 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of 5-Hydroxytryptamine (5-HT) in samples from serum, plasma or other biological fluids. |
5-Hydroxytryptamine (5-HT) ELISA Kit |
DLR-5-HT-Ge-96T |
DL Develop |
96T |
EUR 608 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of 5-Hydroxytryptamine (5-HT) in samples from serum, plasma or other biological fluids. |
S-(5'-Adenosyl)-L-methionine chloride (hydrochloride) |
B2826-5 |
Biovision |
|
EUR 120 |
[D-Ala2]- beta-Casomorphin (1-5), bovine |
5-00075 |
CHI Scientific |
4 x 5mg |
Ask for price |
[D-Pro2]-beta-Casomorphin (1-5) , bovine |
5-00133 |
CHI Scientific |
4 x 5mg |
Ask for price |
beta-Endorphin (1-5) + (16-31), human |
5-00488 |
CHI Scientific |
4 x 1mg |
Ask for price |
Biotin-Kinase Domain of Insulin Receptor (5) |
5-00799 |
CHI Scientific |
4 x 1mg |
Ask for price |
cAMP Dependent PK Inhibitor (5-22), amide |
5-00921 |
CHI Scientific |
4 x 1mg |
Ask for price |
Substance P (5-11)/Hepta-Substance P |
5-01979 |
CHI Scientific |
4 x 5mg |
Ask for price |
5-min. Genomic DNA Prep for PCR |
P930-5 |
101Bio |
- |
Ask for price |
Finally, laccase CLEAs were tested for their capacity to eliminate the known or suspected endocrine disrupting chemicals (EDCs) nonylphenol, bisphenol A and triclosan in a fluidized bed reactor. A 100-ml reactor with 0.5mg of laccase CLEAs operated continuously at a hydraulic retention time of 150min at room temperature and pH 5 could remove all three EDCs from a 5mgl(-1) solution.