Preparation and characterization of cross-linked laccase aggregates and their application to the elimination of endocrine disrupting chemicals.

Laccase from the white rot fungus Coriolopsis polyzona was immobilized for the first time through the formation of cross-linked enzyme aggregates (CLEAs). Laccase CLEAs were produced by using 1000g of polyethylene glycol per liter of enzyme solution as precipitant and 200muM of glutaraldehyde as a cross-linking agent.

These CLEAs had a laccase activity of 148Ug(-1) and an activity recovery of 60.2% when using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as substrate. CLEAs formed by co-aggregation with bovine serum albumin (BSA) as a stabilizer showed lower laccase activity and affinity for ABTS than those without BSA. The CLEAs co-aggregated with BSA showed higher residual activity against a protease, NaN(3), EDTA, methanol and acetone.

The thermoresistance was higher for CLEAs than for free laccase and also higher for CLEAs co-aggregated with BSA than for simple CLEAs when tested at a pH of 3 and a temperature of 40 degrees C.

CHD-5
2693-5
EUR 153

Necrostatin-5
1865-5
EUR 126

GNF-5
B1329-5
EUR 142

Histatin 5
5-01307 4 x 1mg Ask for price

5-Iodotubercidin
A3125-5 5 mg
EUR 168
Description: 5-Iodotubercidin (Itu) is a purine derivative and hence an inhibitor of adenosine kinase with an IC50 value of 26 nM [1]. Adenosine kinase is important in regulating the intracellular and extracellular concentrations of adenosine and hence diverse physiological actions of adenosine [2].

5-ROX
B8212-5 5 mg
EUR 195

Thrombin Receptor Activator for Peptide 5 (TRAP-5)
A1036-5 5 mg
EUR 102
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.

2',5'-Dideoxyadenosine
2770-5
EUR 240

[Cit5]-TRAP-5
5-00062 4 x 5mg Ask for price

A-VI-5
5-00524 4 x 5mg Ask for price

ACTH(5-10)
5-00605 4 x 5mg Ask for price

Angiotensin (1-5)
5-00676 4 x 5mg Ask for price

Bradykinin (1-5)
5-00852 4 x 5mg Ask for price

TRAP-5 amide
5-02019 4 x 5mg Ask for price

Teicoplanin A2-5
C4938-5 5 mg
EUR 1157
Description: Teicoplanin A2-5, derived from Actinoplanes teichomyceticus, is a glycopeptide antibiotic which produces a broad spectrum of antibiotic activity against gram-positive bacteria.

5'-deoxy Thymidine
C4692-5 5 mg
EUR 180
Description: 5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines.

5-fluoro 203
C4114-5 5 mg
EUR 154
Description: 5-fluoro 203 (5F-203) is an antitumor agent and cytotoxic compound that acts as a potent AhR agonist [1][2][3]. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in regulating xenobiotic-metabolizing enzymes such as cytochrome P450.

W-5 hydrochloride
B6277-5 5 mg
EUR 116

5-Nonyloxytryptamine oxalate
B6496-5 5 mg
EUR 154

5-Formylcytosine (5-fC) ELISA Kit
MET-5102-5 5 x 96 assays
EUR 2283

5-Carboxylcytosine (5-caC) ELISA Kit
MET-5103-5 5 x 96 assays
EUR 2283

AICA riboside, 5'-phosphate
9416-5
EUR 153

Individual Reaction Mix 5
G065-5 200 reactions
EUR 167

Necrosis Inhibitor, Necrox-5
2229-5
EUR 615

5-Nitroso-8-quinolinol
B2825-5
EUR 109

Dok- 5 (263 - 275)
5-01048 4 x 5mg Ask for price

Secretin (5 - 27), porcine
5-01917 4 x 1mg Ask for price

SH2 Domain Ligand (5)
5-01929 4 x 5mg Ask for price

Boc-5-Ava-OH
5-04607 25g Ask for price

Boc-5-Ava-OH
5-04608 100g Ask for price

Fmoc-5-Ava-OH
5-04609 5g Ask for price

Fmoc-5-Ava-OH
5-04610 25g Ask for price

FAM azide, 5- isomer
A8117-5 5 mg
EUR 166
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

ROX azide, 5- isomer
A8126-5 5 mg
EUR 583
Description: ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.Pure 5-isomer.

TAMRA azide, 5- isomer
A8129-5 5 mg
EUR 583
Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.TAMRA is often used as FRET acceptor for FAM fluorophore.

5'-(N-Cyclopropyl)carboxamidoadenosine
C4645-5 5 mg
EUR 128

5-trans U-46619
C4261-5 5 mg
EUR 538
Description: 5-trans U-46619 is a minor impurity (2-5%) which variably exists in most commercial preparations of U-46619 [1]. U-46619 is a selective thromboxane A2 agonist involved in inducing platelet aggregation and vascular smooth muscle contraction [2].

2',5'-dideoxy Adenosine
C3993-5 5 mg
EUR 189
Description: 2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1]. Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells.

2-Methyl-5-hydroxytryptamine hydrochloride
2768-5
EUR 185

3,3',5'-Triiodo-L-thyronine
B2017-5
EUR 229

pLenti-CLDN1 shRNA-5 Plasmid
PVTBAV04867-5 2 ug
EUR 356

[Pyr5]-Substance P (5-11)
5-00288 4 x 5mg Ask for price

[Trp3, Arg5]-Ghrelin (1-5)
5-00313 4 x 5mg Ask for price

beta-Casomorphin (1-5) (bovine)
5-00475 4 x 5mg Ask for price

Angiotensin I/II (5-8)
5-00684 4 x 5mg Ask for price

Cholecystokinin Octapeptide (1-5) (desulfated)
5-00970 4 x 5mg Ask for price

HIV-gp41-Antigenic Peptide 5
5-01331 4 x 1mg Ask for price

Fmoc-Trp(5-OH)-OH
5-06939 5g Ask for price

Fmoc-Trp(5-OH)-OH
5-06940 25g Ask for price

Magnetic Beads (DNA) 5 mL
P920-5 - Ask for price

Angiotensin 1/2 (1-5)
A1047-5 5 mg
EUR 119
Description: Angiotensin I/II (1-5) is a peptide (ASP-ARG-VAL-TYR-ILE) that contains the amino acids 1-5 and is converted from Angiotensin I/II.Angiotensin I is formed by the action ofreninonangiotensinogen, an ?-2-globulin with 12 amino acids.

Angiotensin 1/2 (5-7)
A1049-5 5 mg
EUR 119
Description: Angiotensin 1/2 (5-7), (C17H27N5O4), a peptide with the sequence H2N-Ile-His-Pro-OH, MW=365.43. Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure.

3,3',5'-Triiodo-L-thyronine
C5014-5 5 mg
EUR 134
Description: 3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TR? and TR? antagonist [1].

2'-O-Methylpseudouridine-5'-Triphosphate
B7993-.5 5x100 µl (100 mM)
EUR 14023

2'-Fluoro-thymidine-5'-Triphosphate
B8035-.5 5x100 µl (100 mM)
EUR 2059

Helioxanthin derivative 5-4-2
B4700-5 5 mg
EUR 683
Description: Helioxanthin derivative 5-4-2, an analogue of helioxanthin (ACH126447), is a small-molecule inhibitor of HBV, HSV-1and HSV-2 virus with EC50 values of 0.08 ?M, 0.29 ?M and 0.16 ?M, respectively [1].

5?-dihydro-11-keto Testosterone
C4317-5 5 mg
EUR 586
Description: 5?-dihydro-11-keto Testosterone (11KDHT) is a full androgen receptor agonist comparable to dihydrotestosterone (DHT) [1][2][3].

Nickel NTA Magnetic Agarose Bead (5%)
MagNTANi5-5 5ml suspension
EUR 174

beta-Casomorphin (1-5) amide (bovine)
5-00476 4 x 5mg Ask for price

cAMP Dependent PK Inhibitor (5-24)
5-00922 4 x 1mg Ask for price

Kinase Domain of Insulin Receptor (5)
5-01429 4 x 1mg Ask for price

Fmoc-D-Trp(5-Br)-OH
5-04217 1g Ask for price

Fmoc-D-Trp(5-Br)-OH
5-04218 5g Ask for price

Fmoc-D-Trp(5-Cl)-OH
5-04223 1g Ask for price

Fmoc-D-Trp(5-Cl)-OH
5-04224 5g Ask for price

Fmoc-D-Trp(5-F)-OH
5-04227 1g Ask for price

Fmoc-D-Trp(5-F)-OH
5-04228 5g Ask for price

Ac-DL-Trp(5-Br)-OH·DCHA
5-04229 1g Ask for price

Ac-DL-Trp(5-Br)-OH·DCHA
5-04230 5g Ask for price

Ac-DL-Trp(5-Cl)-OH·DCHA
5-04231 1g Ask for price

Ac-DL-Trp(5-Cl)-OH·DCHA
5-04232 5g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid
5-04549 1g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid
5-04550 5g Ask for price

Fmoc-3-Ala(5-Thiazoyl)-OH
5-05361 1g Ask for price

Fmoc-3-Ala(5-Thiazoyl)-OH
5-05362 5g Ask for price

7078D 5 PIPET 5ML STERILE STERILE
7078D-5 25/pk
EUR 217
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

Beta-Sheet Breaker Peptide iA?5
A1005-5 5 mg
EUR 102
Description: Beta-Sheet Breaker Peptide iA?5 (C33H43N5O8), with the sequence H-Leu-Pro-Phe-Phe-Asp-OH, has been shown to inhibit amyloidogenesis in rat brain models.

2'-Fluoro-2'-deoxyadenosine-5'-Triphosphate
B7959-.5 5x100 µl (100 mM)
EUR 2059

2'-Fluoro-2'-deoxycytidine-5'-Triphosphate
B7961-.5 5x100 µl (100 mM)
EUR 2059

2'-Fluoro-2'-deoxyguanosine-5'-Triphosphate
B7962-.5 5x100 µl (100 mM)
EUR 2059

2'-Fluoro-2'-deoxyuridine-5'-Triphosphate
B7963-.5 5x100 µl (100 mM)
EUR 2059

3-bromo-5-phenyl Salicylic Acid
C4516-5 5 mg
EUR 370
Description: Ki: 140 nM for AKR1C1; 1.97 ?M for AKR1C2; 21 ?M for AKR1C3 3-bromo-5-phenyl Salicylic acid is an AKR1C1 inhibitor.

5-bromo-3-phenyl Salicylic Acid
C4610-5 5 mg
EUR 319

5-(N,N-dimethyl)-Amiloride (hydrochloride)
C3505-5 5 mg
EUR 180
Description: 5-(N, N-dimethyl)-Amiloride (hydrochloride), a derivative of amiloride, is an inhibitor of NHE1, NHE2, and NHE3. The Na+/H+ exchanger (NHE) is a protein that has been involved in intracellular pH homeostasis of many mammalian cell types.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)
CBA-155-5 25 preps
EUR 3245
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

5-Hydroxytryptamine (5-HT) ELISA Kit
DLR-5-HT-Ge-48T 48T
EUR 469
Description: A competitive inhibition quantitative ELISA assay kit for detection of 5-Hydroxytryptamine (5-HT) in samples from serum, plasma or other biological fluids.

5-Hydroxytryptamine (5-HT) ELISA Kit
DLR-5-HT-Ge-96T 96T
EUR 608
Description: A competitive inhibition quantitative ELISA assay kit for detection of 5-Hydroxytryptamine (5-HT) in samples from serum, plasma or other biological fluids.

S-(5'-Adenosyl)-L-methionine chloride (hydrochloride)
B2826-5
EUR 120

[D-Ala2]- beta-Casomorphin (1-5), bovine
5-00075 4 x 5mg Ask for price

[D-Pro2]-beta-Casomorphin (1-5) , bovine
5-00133 4 x 5mg Ask for price

beta-Endorphin (1-5) + (16-31), human
5-00488 4 x 1mg Ask for price

Biotin-Kinase Domain of Insulin Receptor (5)
5-00799 4 x 1mg Ask for price

cAMP Dependent PK Inhibitor (5-22), amide
5-00921 4 x 1mg Ask for price

Substance P (5-11)/Hepta-Substance P
5-01979 4 x 5mg Ask for price

DiagNano Silica-Coated Gold Nanoparticles, 5 nm
CG-5 10mL
EUR 719

5-min. Genomic DNA Prep for PCR
P930-5 - Ask for price

Finally, laccase CLEAs were tested for their capacity to eliminate the known or suspected endocrine disrupting chemicals (EDCs) nonylphenol, bisphenol A and triclosan in a fluidized bed reactor. A 100-ml reactor with 0.5mg of laccase CLEAs operated continuously at a hydraulic retention time of 150min at room temperature and pH 5 could remove all three EDCs from a 5mgl(-1) solution.